7YWB

Crystal structure of YTHDF2 in complex with N6-Methyladenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	80.530, 80.530, 113.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.110 - 1.920
R-factor	0.2023
Rwork	0.201
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rdn
RMSD bond length	0.009
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.110	2.030
High resolution limit [Å]	1.920	1.920
Number of reflections	31904	4949
<I/σ(I)>	40.26
Completeness [%]	99.1
Redundancy	12.26
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	2 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2