7YW2
Crystal structure of tRNA 2'-phosphotransferase from Mus musculus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.585, 65.585, 148.459 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.800 - 2.230 |
R-factor | 0.2026 |
Rwork | 0.202 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e3a |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.230 | 74.230 | 2.300 |
High resolution limit [Å] | 2.230 | 8.920 | 2.230 |
Rmerge | 0.050 | 0.027 | 0.567 |
Rmeas | 0.054 | 0.029 | 0.606 |
Rpim | 0.019 | 0.011 | 0.210 |
Total number of observations | 2442 | 13662 | |
Number of reflections | 34775 | 362 | 1693 |
<I/σ(I)> | 22.2 | 52.4 | 3.3 |
Completeness [%] | 100.0 | 99.7 | 99.6 |
Redundancy | 8.3 | 6.7 | 8.1 |
CC(1/2) | 0.999 | 0.999 | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 16% PEG 8000 40 mM Potassium phosphate dibasic 20% Glycerol |