7YVX

Left-handed DNA duplex containing consecutive G-G base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.605
Spacegroup name	P 64 2 2
Unit cell lengths	43.375, 43.375, 71.998
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.560 - 2.990
R-factor	0.2007
Rwork	0.195
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.560	37.560	3.160
High resolution limit [Å]	2.990	9.470	3.000
Rmerge	0.101	0.060	0.318
Rmeas	0.106	0.064	0.335
Number of reflections	1553	54	227
<I/σ(I)>	18.64	25.16	9.82
Completeness [%]	99.7	96.4	100
Redundancy	10.003	8.333	10.282
CC(1/2)	0.998	0.998	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, sodium chloride, hexammine cobalt chloride, sodium cacodylate