7YV3

genetically encoded pH sensor Lime at pH10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	98
Detector technology	PIXEL
Collection date	2019-01-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	109.124, 109.124, 49.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.690 - 2.000
Rwork	0.172
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vcm
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.960	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.049
Number of reflections	23179	2296
<I/σ(I)>	25.69
Completeness [%]	99.9
Redundancy	9.9
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH10, 50% MPD