7YUL
Crystal structure of human BEND6 BEN domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-20 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.092, 39.514, 43.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.110 - 1.820 |
| R-factor | 0.1594 |
| Rwork | 0.158 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yug |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.826 |
| Data reduction software | SAINT (5.8.0267) |
| Data scaling software | SAINT (3.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.110 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.045 | 0.159 |
| Number of reflections | 11195 | 483 |
| <I/σ(I)> | 34.15 | 9.77 |
| Completeness [%] | 99.4 | 92.7 |
| Redundancy | 7.69 | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.12 M Ethylene glycol, 0.1 M Sodium HEPES, 0.1 M MOPS (acid), 12.5% MPD (v/v), 12.5% PEG 1000 (v/v), 12.5% PEG3350 (v/v) |
