7YUI

Crystal structure of HOIL-1L(195-423) in complex with the linear tetra-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL17B
Synchrotron site	NFPSS
Beamline	BL17B
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-06
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	P 21 21 21
Unit cell lengths	58.500, 90.304, 117.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.587 - 2.599
R-factor	0.2052
Rwork	0.203
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7r71 3b08
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.590	2.640
High resolution limit [Å]	2.599	2.600
Rmerge	0.149	1.151
Number of reflections	19702	929
<I/σ(I)>	13.7
Completeness [%]	99.5
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Lithium chloride, 0.1 M Tris(pH 8), 20 %(w/v)PEG 8000