7YTF
Structure of OCPx2 from Nostoc flagelliforme CCNUN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 154.635, 154.635, 86.866 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.680 - 2.650 |
| R-factor | 0.2017 |
| Rwork | 0.199 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xb5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.653 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.960 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.099 | 0.781 |
| Number of reflections | 18058 | 2372 |
| <I/σ(I)> | 29.5 | 5.9 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 39.3 | |
| CC(1/2) | 0.999 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |
