7YT0

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with UDP-galactose from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-08-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.796, 81.514, 146.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 1.760
R-factor	0.1822
Rwork	0.181
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ys8
RMSD bond length	0.003
RMSD bond angle	0.934
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.061	0.714
Rmeas	0.064	0.754
Rpim	0.019	0.232
Total number of observations	695835
Number of reflections	64330	2917
<I/σ(I)>	16.2
Completeness [%]	95.6	87.3
Redundancy	10.8	9.7
CC(1/2)		0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000