7YSZ

Spiroplasma melliferum FtsZ bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97623
Spacegroup name	P 41 21 2
Unit cell lengths	106.546, 106.546, 127.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.161 - 2.300
R-factor	0.180674101114
Rwork	0.178
R-free	0.23716
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vxy
RMSD bond length	0.010
RMSD bond angle	1.079
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.161	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.088	0.691
Number of reflections	33121	3217
<I/σ(I)>	14.9	3.1
Completeness [%]	99.7
Redundancy	8.7
CC(1/2)	0.998	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	1.5M ammonium sulphate, 0.1M Tris pH 8.5