7YST

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with both UDP-glucose and UDP-galactose in chain B from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-03-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.626, 81.385, 146.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.460 - 1.880
R-factor	0.1675
Rwork	0.165
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ys8
RMSD bond length	0.003
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.880	1.880
Rmerge	0.154	1.261
Rmeas	0.160	1.317
Rpim	0.043	0.374
Total number of observations	765291
Number of reflections	54985	2713
<I/σ(I)>	6.1
Completeness [%]	100.0	100
Redundancy	13.9	12.1
CC(1/2)		0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000