7YSA

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) in complex with UDP-glucose in chain A and UDP-galactose in chain B from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-03-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	51.222, 76.452, 79.961
Unit cell angles	90.00, 91.97, 90.00

Refinement procedure

Resolution	28.820 - 1.950
R-factor	0.1691
Rwork	0.167
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ys8
RMSD bond length	0.004
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.106	0.489
Rmeas	0.118	0.573
Rpim	0.052	0.290
Total number of observations	224951
Number of reflections	44546	3852
<I/σ(I)>	8.2
Completeness [%]	98.5	85.8
Redundancy	5	3.3
CC(1/2)		0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000