7YRT

Crystal structure of PAS like domain of FlrB, the histidine kinase involved in flagellar synthesis of Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.972423
Spacegroup name	C 1 2 1
Unit cell lengths	212.644, 49.556, 146.830
Unit cell angles	90.00, 92.53, 90.00

Refinement procedure

Resolution	48.260 - 2.270
R-factor	0.1883
Rwork	0.185
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.260	2.350
High resolution limit [Å]	2.270	2.270
Rmerge	0.046
Number of reflections	69328	6819
<I/σ(I)>	9.1
Completeness [%]	97.7
Redundancy	2.6
CC(1/2)	0.997	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.8 M Ammonium sulfate, 0.1 M Citric Acid pH 5, 10% Glycerol