7YRP
Crystal structure of VIM-28 metallo-beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.360, 45.910, 103.260 |
| Unit cell angles | 90.00, 103.59, 90.00 |
Refinement procedure
| Resolution | 63.483 - 2.000 |
| Rwork | 0.172 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.390 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.730 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.494 |
| Number of reflections | 43247 | 6128 |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.4 M ammonium citrate |
