7YRB
UBR box of human UBR6
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.848, 39.359, 45.248 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.470 - 1.510 |
R-factor | 0.1689 |
Rwork | 0.166 |
R-free | 0.19870 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.063 | 0.834 |
Number of reflections | 11429 | 559 |
<I/σ(I)> | 55.47 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 12.4 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 2.2M Ammonium sulfate, 0.1M HEPES sodium pH 7.5, 1% PEG 400 |