7YPL

Crystal structure of fibronectin type III domain variant, a VEGFR2-specific antagonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-25
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	68.791, 68.791, 68.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.792 - 1.630
Rwork	0.177
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lxo
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	HKL-2000 (2.3.10)
Data scaling software	HKL-2000 (2.3.10)
Phasing software	HKL-3000 (2.3.10)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.630	1.630
Number of reflections	12563	1222
<I/σ(I)>	32.7
Completeness [%]	100.0
Redundancy	10
CC(1/2)	0.895	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Li2SO4 0.1M CHES pH 9.5 0.7M K/Na tartrate