7YPE

Crystal structure of AsfvPCNA in space group of P63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 63
Unit cell lengths	118.660, 118.660, 54.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.660 - 2.200
R-factor	0.2098
Rwork	0.209
R-free	0.22690
Structure solution method	SAD
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.29)
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.660	29.660	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.126	0.039	1.363
Rmeas	0.130	0.040	1.403
Rpim	0.030	0.009	0.332
Total number of observations	410355	6670	34609
Number of reflections	22459	341	1945
<I/σ(I)>	18.9	63.1	2.6
Completeness [%]	100.0	97.4	100
Redundancy	18.3	19.6	17.8
CC(1/2)	0.999	0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 8.5 and 20% (w/v) PEG1000