7YOW
Crystal structure of SARS-CoV-2 omicron variant spike receptor-binding domain (RBD) in complex with NCV2SG48 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.550, 115.682, 99.487 |
| Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
| Resolution | 47.843 - 3.300 |
| R-factor | 0.2108 |
| Rwork | 0.208 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wnb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.013 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.400 |
| Rmerge | 0.094 | 0.699 |
| Number of reflections | 30152 | 4430 |
| <I/σ(I)> | 5.5 | 1.4 |
| Completeness [%] | 97.8 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.995 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.5 M ammonium sulfate, 19.5% PEG 3350, 1 mM EDTA, 10% golycerol, 0.1 M Bis-Tris pH 5.5 |
