7YOP

Spiroplasma melliferum FtsZ bound to GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97623
Spacegroup name	P 41 21 2
Unit cell lengths	106.593, 106.593, 128.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.236 - 2.200
R-factor	0.176964298638
Rwork	0.175
R-free	0.22038
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vxy
RMSD bond length	0.010
RMSD bond angle	1.015
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.236	2.200
High resolution limit [Å]	2.200	2.200
Rmerge	0.068	0.440
Rmeas	0.075	0.485
Rpim	0.031	0.280
Number of reflections	38294	17906
<I/σ(I)>	17	4.3
Completeness [%]	99.5	98.9
Redundancy	5.8	5.6
CC(1/2)	0.998	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.3M sodium acetate, 0.1M Tris pH 8.5, 15% w/v PEG 4000