7YOH
Crystal structure of I88L single mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high-affinity inhibitory peptide from serine acetyltransferase of Haemophilus influenzae at 2.5 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.750, 112.750, 44.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.360 - 2.500 |
| Rwork | 0.243 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y7l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.360 | 2.581 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.790 | |
| Number of reflections | 9865 | 970 |
| <I/σ(I)> | 126.45 | |
| Completeness [%] | 99.5 | 99.69 |
| Redundancy | 15.5 | |
| CC(1/2) | 0.886 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 0.1M HEPES, 1.3M Sodium citrate |
