7YNV

Crystal structure of photolysed Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	78.280, 78.280, 37.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.140 - 1.390
R-factor	0.1757
Rwork	0.174
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ym8
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.140	39.140	1.470
High resolution limit [Å]	1.390	4.170	1.390
Rmerge	0.121	0.109	0.373
Rmeas	0.124	0.111	0.384
Total number of observations	526644
Number of reflections	23873	1009	3508
<I/σ(I)>	23.06	38.8	7.01
Completeness [%]	99.5	99.7	96.8
Redundancy	22.06	22.176	17.839
CC(1/2)	0.998	0.997	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.6	293	28% NaCl, 8% PEG6000, 100 mM Na-acetate (pH3.6)