7YMO
Crystal structure of the recombination mediator protein RecO from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-09-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.147, 61.711, 97.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.690 - 1.800 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.20800 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.775 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 22418 | 1110 |
<I/σ(I)> | 33.1 | 3.9 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 5.7 | 5.6 |
CC(1/2) | 0.995 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3350, sodium formate |