7YLT
Structure of a bacteria protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.775, 75.745, 119.706 |
| Unit cell angles | 85.60, 89.16, 89.37 |
Refinement procedure
| Resolution | 48.670 - 2.300 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.20_4459) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 52101 | 4716 |
| <I/σ(I)> | 9.78 | |
| Completeness [%] | 91.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 291.15 | Polyethylene glycol 3350, Tris, Cobalt(II) chloride hexahydrate |
