7YLG

Crystal structure of the chicken Toll-like receptor 15 TIR domain (glutathione adduct)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-06-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	64.625, 64.625, 94.911
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.980 - 1.800
R-factor	0.2151
Rwork	0.214
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ylf
RMSD bond length	0.009
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	21418	1056
<I/σ(I)>	39.8	2.3
Completeness [%]	97.8	99.5
Redundancy	7.2	7.2
CC(1/2)	0.996	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	Dipotassium hydrogen phosphate, sodium dihydrogen phosphate, sodium acetate