7YLB

Two monobodies recognizing the conserved epitopes of SARS-CoV-2 N antigen applicable to the broad COVID-19 diagnosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	143.081, 48.458, 143.247
Unit cell angles	90.00, 105.16, 90.00

Refinement procedure

Resolution	138.261 - 2.410
R-factor	0.27
Rwork	0.267
R-free	0.32590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yun 1fna
RMSD bond length	0.007
RMSD bond angle	1.675
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	138.261	2.450
High resolution limit [Å]	2.410	2.410
Rmerge	0.186	0.945
Number of reflections	74632	3673
<I/σ(I)>	7.6
Completeness [%]	99.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.2M K2SO4, 18% PEG 3350