7YL6

Cell surface protein YwfG protein complexed with alpha-1,2-mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	43.454, 276.634, 321.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.511 - 2.950
Rwork	0.211
R-free	0.26840
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7yl4
RMSD bond length	0.014
RMSD bond angle	2.033
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	3.110
High resolution limit [Å]	2.950	2.950
Rmerge	0.203	1.187
Rmeas	0.211	1.232
Rpim	0.058	0.329
Number of reflections	42029	6070
<I/σ(I)>	14	2.5
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.996	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate (pH 5.0-5.6), 1.3 M Li2SO4, 2% (w/v) polyethylene glycol 3350