7YKF

Crystal structure of MAGI2 PDZ0-GK/pEphexin4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97737
Spacegroup name	P 1
Unit cell lengths	40.209, 55.616, 61.070
Unit cell angles	65.31, 76.62, 71.56

Refinement procedure

Resolution	29.670 - 2.280
R-factor	0.1883
Rwork	0.186
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eeh 5ypo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.340
High resolution limit [Å]	2.280	6.230	2.280
Rmerge	0.068	0.039	0.317
Rmeas	0.081	0.046	0.380
Rpim	0.043	0.025	0.207
Number of reflections	19920	1004	1005
<I/σ(I)>	6.3
Completeness [%]	97.5	98.4	95.9
Redundancy	3.3	3.4	3.1
CC(1/2)		0.996	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M magnesium chloride hexahydrate, 0.1 M Tris (pH 8.5), 25% w/v PEG 3350