7YK3
Crystal structure of DarTG toxin-antitoxin complex from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97800 |
Spacegroup name | P 1 |
Unit cell lengths | 40.530, 54.550, 93.090 |
Unit cell angles | 105.03, 93.10, 102.69 |
Refinement procedure
Resolution | 34.480 - 2.200 |
R-factor | 0.1823 |
Rwork | 0.179 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7OMY for DarT and RoseTTAFold predicted model for DarG |
RMSD bond length | 0.008 |
RMSD bond angle | 0.900 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (CCP4i 7.0.053) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.480 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.649 |
Rmeas | 0.135 | 0.798 |
Rpim | 0.077 | 0.455 |
Total number of observations | 103813 | 9078 |
Number of reflections | 34943 | 3045 |
<I/σ(I)> | 5.5 | 2.4 |
Completeness [%] | 92.2 | |
Redundancy | 3 | 3 |
CC(1/2) | 0.979 | 0.606 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291.15 | 0.1 M NaHEPES, 20% w/v PEG 10000 |