7YIW

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (ALPL) at Acidic pH

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2021-01-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978530
Spacegroup name	P 21 21 21
Unit cell lengths	159.364, 166.525, 181.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 2.890
R-factor	0.2015
Rwork	0.201
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ew2
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.960	2.993
High resolution limit [Å]	2.890	2.890
Rmerge	0.200
Number of reflections	108466	10707
<I/σ(I)>	12.79
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	0.995	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	20% (w/v) PEG3350, 640 mM sodium acetate, pH5.0