7YIL

The structure of GINS from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	67.410, 67.410, 236.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.670 - 2.170
R-factor	0.2183
Rwork	0.217
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5GHS and 2E9X
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.790	33.790	2.230
High resolution limit [Å]	2.170	9.710	2.170
Rmerge	0.078	0.048	1.576
Rmeas	0.079	0.049	1.603
Rpim	0.013	0.009	0.287
Total number of observations	638608	6685	38718
Number of reflections	17808	265	1269
<I/σ(I)>	26.7	53.9	2.5
Completeness [%]	99.7	97.6	99.7
Redundancy	35.9	25.2	30.5
CC(1/2)	0.999	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20.1% PEG 1500, 5.36% MPD, 0.1M Tris-Base pH8.5