7YH5

MazG(Mycobacterium tuberculosis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	291
Detector technology	CCD
Collection date	2019-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 4 21 2
Unit cell lengths	109.310, 109.310, 242.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.860 - 2.700
R-factor	0.2599
Rwork	0.258
R-free	0.29560
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.860	2.796
High resolution limit [Å]	2.700	2.700
Number of reflections	41350	41350
<I/σ(I)>	17.9
Completeness [%]	99.8
Redundancy	2.8
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 7.5, 10% W/V PEG8000, 8% Ethylene glycol