7YGW

Crystal structure of the Zn2+-bound EFhd1/Swiprosin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.28255
Spacegroup name	P 21 21 21
Unit cell lengths	43.959, 47.393, 63.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.960 - 1.720
R-factor	0.1992
Rwork	0.198
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7clt
RMSD bond length	0.009
RMSD bond angle	0.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.079
Number of reflections	14577	685
<I/σ(I)>	24.4
Completeness [%]	99.5	94.5
Redundancy	12.5
CC(1/2)	1.000	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 2.5 mM ZnSO4, 25% (w/v) Jeffamine ED-2001