7YGV

Crystal structure of the Ca2+-bound EFhd1/Swiprosin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.28255
Spacegroup name	P 21 21 21
Unit cell lengths	44.251, 47.907, 63.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.720 - 2.800
R-factor	0.2004
Rwork	0.195
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7clt
RMSD bond length	0.009
RMSD bond angle	1.039
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge		0.492
Rmeas		0.517
Rpim		0.150
Number of reflections	3596	176
<I/σ(I)>	20	3.7
Completeness [%]	99.8
Redundancy	22
CC(1/2)	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 0.4 mM ZnSO4, and 25% (w/v) Jeffamine ED-2001