7YGK
Crystal structure of a secretory phospholipase A2 from Sciscionella marina
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 47.380, 39.680, 54.300 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 27.140 - 1.240 |
R-factor | 0.17661699011 |
Rwork | 0.175 |
R-free | 0.19716 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1faz |
RMSD bond length | 0.015 |
RMSD bond angle | 1.302 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.290 |
High resolution limit [Å] | 1.240 | 1.240 |
Number of reflections | 28410 | 2762 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.1 | |
Redundancy | 6.4 | |
CC(1/2) | 0.995 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 295.15 | 100 mM citrate pH 5.5, 40% (v/v) PEG 600 |