7YFV

Structure of Rpgrip1l CC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	35.167, 104.674, 38.413
Unit cell angles	90.00, 105.08, 90.00

Refinement procedure

Resolution	29.100 - 2.200
R-factor	0.2163
Rwork	0.214
R-free	0.26310
Structure solution method	AB INITIO PHASING
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Arcimboldo
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.074	0.030	0.807
Rmeas	0.080	0.033	0.874
Rpim	0.030	0.012	0.332
Total number of observations	94590
Number of reflections	13799	707	690
<I/σ(I)>	4.2
Completeness [%]	99.6	99	99.3
Redundancy	6.9	6.4	6.7
CC(1/2)		0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	7.5% w/v PEG6000, 0.1 M Sodium citrate tribasic dihydrate, pH 4.0