7YFU

Structure of Rpgrip1l CC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	81.620, 26.939, 37.469
Unit cell angles	90.00, 90.69, 90.00

Refinement procedure

Resolution	21.160 - 1.500
R-factor	0.1791
Rwork	0.177
R-free	0.21540
Structure solution method	AB INITIO PHASING
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Arcimboldo
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.065	0.048	0.336
Rmeas	0.071	0.053	0.383
Rpim	0.029	0.022	0.177
Total number of observations	77240
Number of reflections	13124	709	551
<I/σ(I)>	9.8
Completeness [%]	98.7	99.4	85.2
Redundancy	5.9	5.7	4.1
CC(1/2)		0.997	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	2.0 M ammonium sulfate