7YF3
Crystal structure of METTL9 in complex with unmethylated S100A9 peptide and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.892, 43.547, 106.492 |
Unit cell angles | 90.00, 123.70, 90.00 |
Refinement procedure
Resolution | 70.319 - 3.434 |
R-factor | 0.2407 |
Rwork | 0.236 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7yf2 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.320 | 3.640 |
High resolution limit [Å] | 3.430 | 3.430 |
Number of reflections | 7067 | 7067 |
<I/σ(I)> | 5.8 | |
Completeness [%] | 94.7 | |
Redundancy | 6.3 | |
CC(1/2) | 0.984 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% (w/v) PEG 4000, 0.1 M NDSB-256 |