7YEW

Structure of MmIGF2BP3 in complex with 7-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	45.890, 73.716, 57.405
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	28.490 - 2.500
R-factor	0.1978
Rwork	0.195
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fqr
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.146	0.872
Rmeas	0.154	0.960
Rpim	0.058	0.395
Number of reflections	12566	1264
<I/σ(I)>	13.69	2
Completeness [%]	96.5	97.2
Redundancy	6.6	5.4
CC(1/2)	0.992	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000