7YEU
Superfolder green fluorescent protein with phosphine unnatural amino acid P3BF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.603, 87.603, 114.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.093 - 1.950 |
| Rwork | 0.160 |
| R-free | 0.19120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.940 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.145 | 1.349 |
| Rmeas | 0.161 | |
| Rpim | 0.070 | 0.469 |
| Number of reflections | 44896 | 2292 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.3 | 99.9 |
| Redundancy | 9.5 | 9.8 |
| CC(1/2) | 0.998 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.2 M Potassium Citrate, 20% PEG3350, pH 8.3 |
