7YEQ

Structural insight into African Swine Fever Virus CP312R protein reveals it as a single-stranded DNA binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	39.172, 66.608, 115.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.770 - 2.320
R-factor	0.218
Rwork	0.214
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.650	2.430
High resolution limit [Å]	2.320	2.320
Rmerge	0.132
Number of reflections	163761	12840
<I/σ(I)>	11.7
Completeness [%]	100.0
Redundancy	12
CC(1/2)	0.998	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.15 M Potassium bromide, and 30% w/v Polyethylene glycol monomethyl ether 2,000