7YDW

Crystal structure of the MPND-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	37.783, 62.922, 69.085
Unit cell angles	72.58, 89.88, 89.98

Refinement procedure

Resolution	33.440 - 2.470
Rwork	0.245
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ydt
RMSD bond length	0.003
RMSD bond angle	1.181
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.470	2.470
Number of reflections	21839	899
<I/σ(I)>	9.5	9.5
Completeness [%]	96.0
Redundancy	3.4
CC(1/2)	0.968	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG4000, sodium citrate, ammonium acetate