7YCO
Crystal structure of SARS-CoV-2 Receptor Binding Domain bound to A6 repebody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-19 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 135.966, 135.966, 69.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 1.960 |
R-factor | 0.2362 |
Rwork | 0.235 |
R-free | 0.25850 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.085 | 1.972 |
Number of reflections | 45636 | 4541 |
<I/σ(I)> | 19.88 | 1.52 |
Completeness [%] | 99.9 | 99.98 |
Redundancy | 13.5 | 14.2 |
CC(1/2) | 0.999 | 0.744 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium citrate tribasic dihydrate, 20 % (w/v) PEG 3350 |