7YC3
Crystal structure of FGFR4 kinase domain with 10t
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-24 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5870 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.333, 61.038, 60.516 |
| Unit cell angles | 90.00, 98.01, 90.00 |
Refinement procedure
| Resolution | 42.762 - 1.987 |
| R-factor | 0.1633 |
| Rwork | 0.162 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wct |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.762 | 2.058 |
| High resolution limit [Å] | 1.987 | 1.987 |
| Rmeas | 0.230 | |
| Number of reflections | 21010 | 1959 |
| <I/σ(I)> | 7.29 | 3.28 |
| Completeness [%] | 99.1 | 92.71 |
| Redundancy | 6.7 | 4.3 |
| CC(1/2) | 0.982 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350 |
