7YC2

Crystal structure of auxiliary protein in complex with human protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	106.300, 72.800, 127.500
Unit cell angles	90.00, 113.40, 90.00

Refinement procedure

Resolution	17.050 - 2.900
R-factor	0.2504
Rwork	0.250
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep0
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.650	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.270
Number of reflections	19906	6197
<I/σ(I)>	4.13
Completeness [%]	98.4	98.2
Redundancy	3.36
CC(1/2)		0.120

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	296	1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic