7YA7
The crystal structure of IpaH1.4 LRR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.467, 68.039, 100.260 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.18712 |
| Rwork | 0.185 |
| R-free | 0.21984 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b0n |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.502 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.062 | 0.487 |
| Number of reflections | 86206 | 8222 |
| <I/σ(I)> | 33.9 | 4 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 50 mM imidazole (pH 8.0), 1.2 M NaCl. |
