7Y9B
Crystal structure of the membrane (M) protein of a SARS-COV-2-related coronavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.569, 66.571, 112.628 |
| Unit cell angles | 90.00, 109.83, 90.00 |
Refinement procedure
| Resolution | 37.090 - 3.214 |
| R-factor | 0.2719 |
| Rwork | 0.271 |
| R-free | 0.29310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7y96 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.412 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.878 | 48.878 | 3.252 |
| High resolution limit [Å] | 3.194 | 8.904 | 3.194 |
| Rmerge | 0.147 | 0.035 | 0.697 |
| Rmeas | 0.175 | 0.043 | 0.835 |
| Rpim | 0.094 | 0.025 | 0.453 |
| Total number of observations | 56567 | 2580 | 2692 |
| Number of reflections | 16908 | 844 | 846 |
| <I/σ(I)> | 6.9 | 19.3 | 1.6 |
| Completeness [%] | 94.1 | 95.6 | 91.9 |
| Redundancy | 3.3 | 3.1 | 3.2 |
| CC(1/2) | 0.994 | 0.998 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.8 | 293 | 300 mM ammonium formate, 50 mM Tris-HCl pH 8.8, 35% PEG 500 MME, 7.1 mM pentaethylene glycol monooctyl ether |
