7Y8H
Crystal structure of sDscam FNIII1 domain, isoform alpha7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.977, 43.211, 48.338 |
| Unit cell angles | 90.00, 111.29, 90.00 |
Refinement procedure
| Resolution | 38.190 - 1.800 |
| R-factor | 0.2255 |
| Rwork | 0.223 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1va9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 14730 | 939 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.970 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% (w/v) PEG 1,500, 100 mM SPG buffer pH 5.5 |
