7Y7O
Crystal structure of metallo-endoribonuclease YbeY from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-08-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.28176 |
| Spacegroup name | P 63 |
| Unit cell lengths | 100.663, 100.663, 50.187 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.590 - 1.900 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.22560 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.184 |
| Rmeas | 0.055 | 0.194 |
| Rpim | 0.012 | 0.044 |
| Number of reflections | 22303 | 1017 |
| <I/σ(I)> | 56 | 14.6 |
| Completeness [%] | 96.6 | 88.7 |
| Redundancy | 21.1 | 18.8 |
| CC(1/2) | 1.000 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 0.1 M Sodium citrate (pH 5.5), 40% (v/v) PEG 400, 1 mM Zinc sulfate |
