7Y6B
Crystal structure of the EscE/EsaH complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.550, 52.750, 65.510 |
| Unit cell angles | 90.00, 114.03, 90.00 |
Refinement procedure
| Resolution | 39.568 - 1.800 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.22830 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.380 |
| Rmeas | 0.092 | 0.495 |
| Rpim | 0.063 | 0.345 |
| Number of reflections | 31956 | 4667 |
| <I/σ(I)> | 9 | 2.8 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.995 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 0.1 M KSCN, 0.1 M Tris pH 8.4, 23% Polyethylene glycol monomethyl ether 2000, 3%(w/v) 1,6-Hexanediol |
