7Y62

Crystal structure of human TFEB HLHLZ domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	54.600, 54.600, 185.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.280 - 2.000
R-factor	0.2223
Rwork	0.222
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d8s
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.280	47.280	2.050
High resolution limit [Å]	2.000	8.950	2.000
Rmerge	0.056	0.024	1.533
Rmeas	0.058	0.025	1.573
Rpim	0.014	0.006	0.352
Total number of observations	417639	4585	32151
Number of reflections	22446	322	1628
<I/σ(I)>	27.2	68	2.1
Completeness [%]	99.2	99.9	100
Redundancy	18.6	14.2	19.7
CC(1/2)	1.000	1.000	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Ethylene glycols, Buffer system 3, and Precipitant Mix 2