7Y4R

Structure of RclX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	78.170, 92.710, 96.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.610 - 2.510
R-factor	0.2095
Rwork	0.206
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dip
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.143	0.074	0.376
Rmeas	0.152	0.077	0.423
Rpim	0.050	0.022	0.185
Total number of observations	174870
Number of reflections	23999	1362	972
<I/σ(I)>	6.5
Completeness [%]	96.7	99.2	80.4
Redundancy	7.3	11.4	3.8
CC(1/2)		0.998	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.1 M sodium acetate trihydrate pH 4.6 15.5% (v/v) PEG 300